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The research group coordinated by Prof. Daniela Cecconi has a specific
expertise and a strong technical background in proteome analysis, a field in
which Prof. Cecconi pioneered some of the techniques presently in use (such as isotopic labelling
for differential proteomic analysis [1], multivariate statistical approach for
2DE data analysis [2-6], combinatorial peptide ligand libraries for protein prefractionation
[7]).
The main research activity of
the laboratory is the study of the Pancreatic Cancer through Proteomic technologies.
This research is active in the laboratory since 2003 [8-14] (thanks to the
collaboration with research groups of Medicine, mainly Prof. Scarpa and Prof.
Palmieri).
Currently the group is working on a project concerning shotgun proteomic
analysis of pancreatic cancer stem cells, which is also based on a collaboratioon with the NIHR
Liverpool Pancreas Biomedical Research Unit, University of Liverpool (Prof.
Costello).
Since 2013 Daniela Cecconi is also involved
in a COST Action, EU Pancreas, as an Expert member for Italy of the COST
Action (BM1204), “An integrated European platform for pancreas cancer research:
from basic science to clinical and public health interventions for a rare
disease”.
Representative Publications:
1) "Quantitative analysis of two-dimensional gel-separated proteins using
isotopically marked alkylating agents and matrix-assisted laser
desorption/ionization mass spectrometry" Gehanne, S., Cecconi, D.,
Carboni, L., Righetti, P.G., Domenici, E., Hamdan, M., Rapid Commun. Mass
Spectrom 2002, 16: 1692-1698.
2) "A new integrated statistical approach to the diagnostic use of
two-dimensional maps" Marengo, E., Robotti, E., Gianotti, V., Righetti,
P.G., Cecconi, D., Domenici, E., Electrophoresis 2003, 24: 225-236.
3) "Application of three-way principal component analysis to the
evaluation of two-dimensional maps in proteomics" Marengo, E., Leardi, R.,
Robotti, E., Righetti, P.G., Antonucci, F., Cecconi, D., J. Proteome Research
2003, 2: 351-360.
4) Identification of the regulatory proteins in human pancreatic cancers
treated with Trichostatin-A by 2D-PAGE maps and Multivariate Statistical
Analysis" Marengo, E., Robotti, E., Cecconi, D., Hamdan, M., Scarpa, A.,
Righetti, P.G., Anal. Bioanal. Chemistry 2004, 379: 992-1003.
5) "Numerical approaches for quantitative analysis of 2D map: a review of
commercial software and of "home-made" systems" Marengo E.,
Robotti E., Antonucci F., Cecconi D., Campostrini N. and Righetti P.G.,
Proteomics 2005, 5: 654-666.
6) "Application of Partial Least Squares Discriminant Analysis and
variable selection procedures: a 2D-PAGE Proteomic-Based Study" Marengo,
E., Robotti, E., Bobba, M., Milli, A., Campostrini, N., Righetti, S.C., Cecconi
D. and Righetti P.G., Anal. Bioanal. Chemistry 2008, 390: 1327-42.
7) "Exploring the hidden human urinary proteome via ligand library
beads" Castagna, A.*, Cecconi, D.*, Sennels, L., Rappsilber, J., Guerrier,
L., Fortis, F., Boschetti, E., Lomas, L., Righetti, P.G., J. Proteome Research
2005, 4: 1917-1930. *contributed equally and share the first authorship.
8) "Proteomic profiling of pancreatic ductal
carcinoma cell lines treated with trichostatin-A" Cecconi, D., Scarpa, A.,
Donadelli, M., Palmieri, M., Hamdan, M., Astner, H., Righetti, P.G.,
Electrophoresis 2003, 24: 1871-1878.
9) "Proteomic analysis of pancreatic ductal carcinoma cells treated with
5-aza-2'-deoxycytidine (DAC)" Cecconi, D., Astner, H., Donadelli, M.,
Palmieri, M., Missiaglia, E., Hamdan, M., Scarpa, A., Righetti P.G.
Electrophoresis 2003, 24: 4291-4303.
10) "Proteomic analysis of pancreatic ductal carcinoma cells after
combined treatment with gemcitabine and trichostatin A" Cecconi, D.,
Donadelli, M., Scarpa, A., Milli, A., Palmieri, M., Hamdan, M., Rappsilber, J.,
Areces, L.B., Righetti, P.G., J. Proteome Research 2005, 4: 1900-1916.
11) "Proteomic analysis of pancreatic endocrine tumour cell lines treated
with the histone deacetylase inhibitor trichostatin A" Cecconi, D.,
Donadelli, M., Rinalducci, S., Zolla, L., Scarpa, A., Palmieri, M., Righetti,
P.G., Proteomics 2007, 7: 1644-1653.
12) "Synergistic effect of trichostatin A and 5-aza-2'-deoxycytidine on
growth inhibition of pancreatic endocrine tumour cell lines: A proteomic
study." Cecconi D, Donadelli M,
Dalla Pozza E, Rinalducci S, Zolla L, Scupoli MT, Righetti PG, Scarpa A,
Palmieri M. Proteomics 2009; 9: 1952-66.
13) “Proteomics in
pancreatic cancer research” Cecconi D, Palmieri M, Donadelli M. Proteomics. 2011
Feb;11(4):816-28.
14) “Comparative proteomic and phosphoproteomic profiling of pancreatic
adenocarcinoma cells treated with CB1 or CB2 agonists” Brandi J, Dando I,
Palmieri M, Donadelli M, Cecconi D. Electrophoresis. 2013 May;34(9-10):1359-68.
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